The publication describes an on-the-fly tracking experiment using a self tracking interferometer on a small three-axis machining center. On-the-fly based on continuous measurement, you can get much more measured points while reducing the time required for machine tool calibration than using Trigger or time measurements. The present publication compares the time required for calibration of the machine, and compares measured and evaluated data with each other. At the end the results are presented for calibration and verification of volumetric compensation with self tracking interferometer. In addition, the results obtained were verified by a circular test ISO 230-4.