This paper deals with vibration analysis of a V-Belt drive based on altering conditions of pulleys misalignment. The main question this paper tries to answer is whether there is a direct and measurable relation between the pulley misalignment conditions and vibration of the machine. Using the ADASH vibration analyser with accelerometer sensors and laser speed sensor attached, we gathered data from our test stand purposely build for this task. Designing of the test stand is described as well as some basics of vibration measurement and belt drive operation. Next a testing methodology is described where several conditions of parallel and angular misalignment as well as different belt tension conditions were simulated using adjustability of our testing stand. Then in data analysis part we look at relations between multiple measurements of different pulley misalignment conditions viewed in time domain vibration analysis. [VSB-TUO].