Measuring the potentially adverse effects of materials on the human body is vital and essential for their use, including medical applications. One of the properties that need to be known is the cytotoxic effect of the materials to avoid local and systemic adverse effects. It is very important that scientists and medical professionals are aware of the effects of materials on the human body. This study also summarizes the data on the morphology, chemical composition, and the effect of ethylene-vinyl acetate (EVA) orthopaedic materials on cytotoxicity. Qualitative and quantitative cytotoxicity analysis was performed using fluorescent microscopy and metabolic cell toxicity assay. The results of the studies demonstrated low in vitro cytotoxicity and high biocompatibility. These results may serve as the basis for a toxicological study of orthopaedic materials to prevent severe side effects.