Agile methods, a perspective alternative to the traditional project management, extend across organisations gradually. Their introduction is, however, confronted with safety aspects which are an integral part of activities in each organisation. This article deals with the relation between agile methods and the safety culture of an organisation using a comparative study based on authors’ research performed in the Czech Republic. It presents fundamental safety aspects, which must be respected while using agile methods and how they can be reflected namely in the agile method Scrum. The article aims to explore the prerequisites for a symbiotic functioning of agile methods and safety culture and in this way to help improve the competitiveness of organisations.