This article deals with the mechanical testing of polymeric materials processed using 3D printing and exposed to different chemical compounds. Information on this resistance is very important for the user from the point of view of the multidisciplinary use of prototypes of 3D printers for various applications. Materials used for these purposes are processed using the photopolymer processing technology PolyJet Matrix technology and thermoplastics processed using FDM technology. Testing was conducted in accordance with the international standards ČSN EN ISO 175 and ČSN EN ISO 527. The conclusion of the article describes an evaluation of the mechanical and physical properties of the materials tested before and after chemical loading.