The aim of this work is to investigate anti-reflective coatings for surface matting and their effect on optical sensing accuracy. In the experimental part, the thickness of the coatings is analyzed on samples with different surface roughness levels: Ra 0.2, Ra 1.7, and Ra 3.1. The samples were milled from C45 steel. Seven types of matting sprays (sublimation and permanent) were tested. The results show that the ATTBLIME AB P spray achieved the lowest average coating thickness of 0.4 µm with a minimal uncertainty of 1 µm for Ra 0.2, making it the most reliable for thin layers. ATTBLIME AB ZERO spray, in contrast, had an average thickness of 19 µm with a higher uncertainty of 17 µm for Ra 1.7. Only ATTBLIME AB P and Helling sprays met the manufacturer's declared parameters across all samples. Permanent sprays, especially ATTBLIME AB P, best suit thin matting layers on glossy surfaces.